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Predicting myofiber size with electrical impedance myography: A study in immature mice
Author(s) -
Kapur Kush,
Taylor Rebecca S.,
Qi Kristin,
Nagy Janice A.,
Li Jia,
Sanchez Benjamin,
Rutkove Seward B.
Publication year - 2018
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.26111
Subject(s) - electrical impedance myography , myocyte , biomedical engineering , electrical impedance , muscle fibre , anatomy , medicine , cardiology , physics , skeletal muscle , quantum mechanics , vasodilation
: Electrical impedance can be used to estimate cellular characteristics. We sought to determine whether it could be used to approximate myofiber size using standard prediction modeling approaches. Methods : Forty‐four C57BL/6J wild‐type immature mice of varying ages underwent electrical impedance myography (EIM) with a needle electrode array placed in the gastrocnemius. Animals were then humanely killed and muscle fixed, stained, and myofiber size quantified. Two different statistical prediction models were then applied. Results : Impedance parameters showed major variation with increasing myofiber size. The prediction models based on EIM data alone were able to predict fiber size, with errors in the range of ±69.05–78.44 µm 2 (16.19%–18.40% with respect to the average myofiber size). Discussion : By using well‐established statistical models, EIM data alone can provide a satisfactory estimate of myofiber size. Additional study of this approach for approximating myofiber size without the requirement of removing tissue for histological analysis is warranted. Muscle Nerve 58 : 106–113, 2018