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Monitoring of macrophage recruitment enhanced by Toll‐like receptor 4 activation with MR imaging in nerve injury
Author(s) -
Chen MeiWei,
Zhang Xiang,
Lu LieJing,
Zhang Fang,
Duan XiaoHui,
Zheng ChuShan,
Chen YueYao,
Shen Jun
Publication year - 2018
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.26097
Subject(s) - tlr4 , sciatic nerve injury , medicine , sciatic nerve , nerve injury , lipopolysaccharide , macrophage , magnetic resonance imaging , saline , toll like receptor , pathology , intraperitoneal injection , anesthesia , receptor , chemistry , radiology , innate immune system , biochemistry , in vitro
: Macrophage recruitment is critical for nerve regeneration after an injury. The aim of this study was to investigate whether ultrasmall superparamagnetic iron oxide (USPIO) nanoparticle‐based MRI could be used to monitor the enhanced macrophage recruitment by Toll‐like receptor 4 (TLR4) activation in nerve injury. Methods : Rats received intraperitoneal injections of either lipopolysaccharide (LPS) or phosphate buffered saline (PBS) or no injection (controls) after a sciatic nerve crush injury. After intravenous injection of the USPIOs (LPS and PBS groups) or PBS (control group), MRI was performed and correlated with histological findings. Results : LPS group showed more remarkable hypointense signals on T2*‐weighted imaging and lower T2 values in the crushed nerves than PBS group. The hypointense signal areas were associated with an enhanced recruitment of iron‐loaded macrophages to the injured nerves. Discussion : USPIO‐enhanced MRI can be used to monitor the enhanced macrophage recruitment by means of TLR4 signal pathway activation in nerve injury. Muscle Nerve 58 : 123–132, 2018