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Nerve growth factor and basic fibroblast growth factor promote reinnervation by nerve−muscle−endplate grafting
Author(s) -
Mu Liancai,
Sobotka Stanislaw,
Chen Jingming,
Nyirenda Themba
Publication year - 2018
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.25726
Subject(s) - reinnervation , nerve growth factor , motor endplate , medicine , anatomy , basic fibroblast growth factor , neuromuscular junction , endocrinology , growth factor , biology , neuroscience , receptor
This study was designed to test whether exogenous application of nerve growth factor (NGF) and basic fibroblast growth factor (FGF‐2) to muscles reinnervated with nerve−muscle−endplate band grafting (NMEG) could promote specific outcomes. Methods The right sternomastoid muscle in adult rats was experimentally denervated and immediately reinnervated by implanting an NMEG pedicle from the ipsilateral sternohyoid muscle. A fibrin sealant containing NGF and FGF‐2 was focally applied to the implantation site. Maximal tetanic force, muscle weight, regenerated axons, and motor endplates were analyzed 3 months after treatment. Results Mean tetanic force, wet muscle weight, and number of regenerated axons in the treated muscles were 91%, 92%, and 84% of the contralateral controls, respectively. The majority of endplates (86%) in the treated muscles were reinnervated by regenerated axons. Discussion Focal administration of NGF and FGF‐2 promotes efficacy of the NMEG technique. Muscle Nerve 57 : 449–459, 2018