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Effect of calcitonin on cultured schwann cells
Author(s) -
Yan JiGeng,
Zhang LinLing,
Agresti Michael A.,
Shen Fengyi,
Matloub Hani S.,
Yan Yuhui,
Li Jifeng,
Gu YuDong,
Logiudice John A.,
Havlik Robert
Publication year - 2017
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.25519
Subject(s) - calcitonin , calcium , schwann cell , propidium iodide , medicine , endocrinology , chemistry , sciatic nerve , calcium metabolism , microbiology and biotechnology , biology , apoptosis , biochemistry , programmed cell death
: After nerve injury, calcium concentrations in intranerve fibers quickly increase. We have shown that functional recovery of injured nerves correlates with calcium absorption. A slight increase in calcium reduces the number of Schwann cells present. Calcitonin therapy greatly improves regeneration by accelerating calcium absorption. We examined the effect of adding calcitonin to higher concentration calcium media on cultured Schwann cells. Methods : The cells, isolated from intact sciatic nerves, were cultured with normal or higher concentration calcium media with or without calcitonin. Schwann cells were incubated with anti–S‐100, goat–anti‐mouse, and propidium iodide and then viewed through fluorescent light and phase‐contrast microscopy for observation and analysis. Results : The cells in each calcitonin‐containing medium showed many Schwann cells, however, the cells in the higher concentration calcium media showed fewer and more defective Schwann cells. Conclusion : These results show that calcitonin protects against the harmful effects of excessive calcium encountered in peripheral nerve injury. Muscle Nerve 56 : 768–772, 2017