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Neuromuscular transmission and muscle fatigue changes by nanostructured oxygen
Author(s) -
Ivannikov Maxim V.,
Sugimori Mutsuyuki,
Llinás Rodolfo R.
Publication year - 2017
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.25248
Subject(s) - neuromuscular transmission , isometric exercise , neurotransmission , neuromuscular junction , synaptic vesicle , electrophysiology , muscle fatigue , biophysics , myocyte , chemistry , anatomy , neuroscience , electromyography , biology , medicine , vesicle , receptor , biochemistry , membrane
: Oxygen (O 2 ) nanobubbles offer a new method for tissue oxygenation. The effects of O 2 nanobubbles on transmission at neuromuscular junctions (NMJs) and muscle function were explored in murine diaphragm. Methods : Electrophysiological parameters, NMJ ultrastructure, muscle force, and muscle fatigue were studied during superfusion with solutions with different oxygen levels or oxygen nanobubbles. Results : High frequency nerve stimulation of muscles superfused with O 2 nanobubble solution slowed neurotransmission decline over those with either control or hyperoxic solution. O 2 nanobubble solution increased the amplitude of evoked end plate potentials and quantal content but did not affect spontaneous activity. Electron microscopy of stimulated O 2 nanobubble treated NMJs showed accumulation of large synaptic vesicles and endosome‐like structures. O 2 nanobubble solution had no effects on isometric muscle force, but it significantly decreased fatigability and maximum force recovery time in nerve stimulated muscles. Conclusions : O 2 nanobubbles increase neurotransmission and reduce the probability of neurotransmission failure in muscle fatigue. Muscle Nerve 55 : 555–563, 2017