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Inflammatory response during slow‐ and fast‐twitch muscle regeneration
Author(s) -
Zimowska Malgorzata,
Kasprzycka Paulina,
Bocian Katarzyna,
Delaney Kamila,
Jung Piotr,
Kuchcinska Kinga,
Kaczmarska Karolina,
Gladysz Daria,
Streminska Wladyslawa,
Ciemerych Maria Anna
Publication year - 2017
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.25246
Subject(s) - proinflammatory cytokine , soleus muscle , inflammation , regeneration (biology) , skeletal muscle , extensor digitorum longus muscle , fibrosis , muscle tissue , anatomy , medicine , chemistry , endocrinology , biology , microbiology and biotechnology
ABSTRACT Introduction : Skeletal muscles are characterized by their unique ability to regenerate. Injury of a so‐called fast‐twitch muscle, extensor digitorum longus (EDL), results in efficient regeneration and reconstruction of the functional tissue. In contrast, slow‐twitch muscle (soleus) fails to properly reconstruct and develops fibrosis. This study focuses on soleus and EDL muscle regeneration and associated inflammation. Methods : We determined differences in the activity of neutrophils and M1 and M2 macrophages using flow cytometry and differences in the levels of proinflammatory cytokines using Western blotting and immunolocalization at different times after muscle injury. Results : Soleus muscle repair is accompanied by increased and prolonged inflammation, as compared to EDL. The proinflammatory cytokine profile is different in the soleus and ED muscles. Conclusions : Muscle repair efficiency differs by muscle fiber type. The inflammatory response affects the repair efficiency of slow‐ and fast‐twitch muscles. Muscle Nerve 55 : 400–409, 2017