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Vitamin D and/or calcium deficient diets may differentially affect muscle fiber neuromuscular junction innervation
Author(s) -
Gifondorwa David J.,
Thompson Tyran D.,
Wiley June,
Culver Alexander E.,
Shetler Pamela K.,
Rocha Guilherme V.,
Ma Yanfei L.,
Krishnan Venkatesh,
Bryant Henry U.
Publication year - 2016
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.25146
Subject(s) - neuromuscular junction , biology , endocrinology , medicine , vitamin d and neurology , skeletal muscle , soleus muscle , calcium , synapse , neuroscience
: There is evidence that supports a role for Vitamin D (Vit. D) in muscle. The exact mechanism by which Vit. D deficiency impairs muscle strength and function is not clear. Methods : Three‐week‐old mice were fed diets with varied combinations of Vit. D and Ca 2+ deficiency. Behavioral testing, genomic and protein analysis, and muscle histology were performed with a focus on neuromuscular junction (NMJ) ‐related genes. Results : Vit. D and Ca 2+ deficient mice performed more poorly on given behavioral tasks than animals with Vit. D deficiency alone. Genomic and protein analysis of the soleus and tibialis anterior muscles revealed changes in several Vit. D metabolic, NMJ‐related, and protein chaperoning and refolding genes. Conclusions : These data suggest that detrimental effects of a Vit. D deficient or a Vit. D and Ca 2+ deficient diet may be a result of differential alterations in the structure and function of the NMJ and a lack of a sustained stress response in muscles. Muscle Nerve 54 : 1120–1132, 2016