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Skeletal muscle fiber characteristics and oxidative capacity in hemiparetic stroke survivors
Author(s) -
Severinsen Kaare,
Dalgas Ulrik,
Overgaard Kristian,
Pedersen Asger R.,
øRtenblad Niels,
Lund Caroline,
Jakobsen Johannes K.,
Andersen Henning
Publication year - 2016
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.24907
Subject(s) - citrate synthase , skeletal muscle , stroke (engine) , medicine , endocrinology , oxidative phosphorylation , muscle fibre , myopathy , fiber type , anatomy , chemistry , pathology , enzyme , biochemistry , mechanical engineering , engineering
: Skeletal muscle is changed after stroke, but conflicting data exist concerning muscle morphology and oxidative enzyme capacity. Methods : In 36 chronic stroke patients bilateral rectus femoris muscle biopsies were analyzed, and fiber type proportions and cross‐sectional areas were determined by ATPase histochemistry. Enzymatic concentrations of citrate synthase (CS) and 3‐Hydroxyacyl‐coenzymeA‐dehydrogenase (HAD) were determined using freeze‐dried muscle tissue. Findings were correlated with clinical outcomes. Results : In the paretic muscles the mean fiber area was smaller (P = 0.0004), and a lower proportion of type 1 fibers (P = 0.0016) and a higher proportion of type 2X fibers (P = 0.0002) were observed. The paretic muscle had lower CS (P = 0.013) and HAD concentrations (P = 0.037). Mean fiber area correlated with muscle strength (r = 0.43; P = 0.041), and CS concentration correlated with aerobic capacity (r = 0.47; P = 0.01). Conclusions : In stroke survivors there is a phenotypic shift toward more fatigable muscle fibers with reduced oxidative enzymatic capacity that relates to clinical outcomes. Muscle Nerve 53 : 748–754, 2016