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Expression of intercellular adhesion molecule‐1 by myofibers in mdx mice
Author(s) -
TorresPalsa Maria J.,
Koziol Matthew V.,
Goh Qingnian,
Cicinelli Peter A.,
Peterson Jennifer M.,
Pizza Francis X.
Publication year - 2015
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.24626
Subject(s) - duchenne muscular dystrophy , mdx mouse , western blot , muscular dystrophy , intercellular adhesion molecule 1 , biology , cell adhesion molecule , intracellular , immunofluorescence , microbiology and biotechnology , intercellular adhesion molecule , skeletal muscle , blot , dystrophin , pathology , cell adhesion , anatomy , immunology , antibody , medicine , cell , biochemistry , gene , genetics
: We investigated the extent to which intercellular adhesion molecule‐1 (ICAM‐1), a critical protein of the inflammatory response, is expressed in skeletal muscles of mdx mice (a murine model of Duchenne muscular dystrophy). Methods : Muscles were collected from control and mdx mice at 2‐24 weeks of age and analyzed for ICAM‐1 expression by means of Western blot and immunofluorescence. Results : Western blot revealed higher expression of ICAM‐1 in mdx compared with control muscles through 24 weeks of age. In contrast to control muscles, ICAM‐1 was expressed on the membrane of damaged, regenerating, and normal myofibers of mdx mice. CD11b+ myeloid cells also expressed ICAM‐1 in mdx muscles, and CD11b+ cells were closely associated with the membrane of myofibers expressing ICAM‐1. Conclusions : These findings support a paradigm in which ICAM‐1 and its localization to myofibers in muscles of mdx mice contributes to the dystrophic pathology. Muscle Nerve 52: 795–802, 2015