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Effects of tail suspension on serum testosterone and molecular targets regulating muscle mass
Author(s) -
Naeyer Hélène,
Lamon Séverine,
Russell Aaron P.,
Everaert Inge,
Spaey Annelies,
Jamart Cécile,
Vanheel Bert,
Taes Youri,
Derave Wim
Publication year - 2015
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.24542
Subject(s) - endocrinology , muscle atrophy , medicine , testosterone (patch) , downregulation and upregulation , muscle hypertrophy , anabolism , atrophy , myostatin , androgen , skeletal muscle , chemistry , soleus muscle , hormone , gene , biochemistry
: The contribution of reduced testosterone levels to tail suspension (TS)‐induced muscle atrophy remains equivocal. The molecular mechanism by which testosterone regulates muscle mass during TS has not been investigated. Methods : Effects of TS on serum testosterone levels, muscle mass, and expression of muscle atrophy‐ and hypertrophy‐inducing targets were measured in soleus (SOL) and extensor digitorum longus (EDL) muscles after testosterone administration during 1, 5, and 14 days of TS in male mice. Results : TS produced an increase followed by a transient drop in testosterone levels. Muscle atrophy was associated with downregulation of Igf1 and upregulation of Mstn , Redd1 , Atrogin‐1 , and MuRF1 mRNA with clear differences in Igf1 , Mstn , and MAFbx/Atrogin‐1 gene expression between SOL and EDL. Testosterone supplementation did not affect muscle mass or protein expression levels during TS. Conclusions The known anabolic effects of testosterone are not sufficient to ameliorate loss of muscle mass during TS. Muscle Nerve 52 : 278–288, 2015