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Electrical impedance myography for the in vivo and ex vivo assessment of muscular dystrophy ( mdx ) mouse muscle
Author(s) -
Li Jia,
Geisbush Tom R.,
Rosen Glenn D.,
Lachey Jennifer,
Mulivor Aaron,
Rutkove Seward B.
Publication year - 2014
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.24086
Subject(s) - electrical impedance myography , mdx mouse , duchenne muscular dystrophy , muscular dystrophy , ex vivo , dystrophin , medicine , in vivo , connective tissue , biomarker , neuromuscular disease , pathology , disease , biology , biochemistry , microbiology and biotechnology , vasodilation
Abstract Introduction : Sensitive, non‐invasive techniques are needed that can provide biomarkers of disease status and the effects of therapy in muscular dystrophy. Methods : We evaluated electrical impedance myography (EIM) to serve in this role by studying 2‐month‐old and 18‐month‐old mdx and wild‐type (WT) animals (10 animals in each of 4 groups). Results : Marked differences were observed in EIM values between mdx and WT animals; the differences were more pronounced between the older age groups (e.g., reactance of 92.6 ±4.3 Ω for mdx animals vs. 130 ± 4.1 Ω for WT animals, P < 0.001). In addition, in vivo EIM parameters correlated significantly with the extent of connective tissue deposition in the mdx animals. Conclusions : EIM has the potential to serve as a valuable non‐invasive method for evaluating muscular dystrophy. It can be a useful biomarker to assist with therapeutic testing in both pre‐clinical and clinical studies. Muscle Nerve 49 : 829–835, 2014