Premium
Reg3G gene expression in regenerating skeletal muscle and corresponding nerve
Author(s) -
Klasan Gordana Starcevic,
Ivanac Danijel,
Erzen Dubravka Jurisic,
Picard Anne,
Takasawa Shin,
Peharec Stanislav,
Arbanas Juraj,
Girotto Dean,
Jerkovic Romana
Publication year - 2014
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.23877
Subject(s) - skeletal muscle , regeneration (biology) , soleus muscle , gene expression , anatomy , biology , western blot , extensor digitorum longus muscle , gene , microbiology and biotechnology , genetics
: The Reg genes play a major role in the regeneration of various tissues; however, no reports have been published regarding expression of the Reg3G gene in skeletal muscle. In this study we investigated the expression of the Reg3G gene in regeneration of rat skeletal muscle and injured nerves. Methods : We used 3 experimental models of muscle and nerve injury. RT‐PCR and Western blot analysis were performed for detection of Reg3G in regenerating muscle and nerve. Results : We found transcriptional activation of the Reg3G gene in the soleus and extensor digitorum longus muscles and in their corresponding nerves after both muscle and nerve injury in different time periods, respectively. Conclusions : The results suggest that the Reg3G gene plays a major role in communication between injured axons and muscle and may play a significant role in skeletal muscle and peripheral nerve regeneration. Muscle Nerve 49 : 61–68, 2014