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Reduced DHPRα1S and RyR1 expression levels are associated with diaphragm contractile dysfunction during sepsis
Author(s) -
Jiao GuangYu,
Hao LiYing,
Gao ChunE,
Chen Lie,
Sun XueFei,
Yang HuaLi,
Li Ying,
Dai YiNing
Publication year - 2013
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.23805
Subject(s) - ryr1 , ryanodine receptor , isometric exercise , sepsis , diaphragm (acoustics) , medicine , contraction (grammar) , ryanodine receptor 2 , muscle contraction , endocrinology , skeletal muscle , receptor , biology , physics , acoustics , loudspeaker
Sepsis often causes diaphragm contractile dysfunction. Dihydropyridine receptors (DHPRα1s and DHPRα1c) and ryanodine receptors (RyR1, RyR2, and RyR3) are essential for excitation–contraction coupling in striated muscles. However, their expression in diaphragm during sepsis have not been explored. Methods : Eight rats received endotoxin, and 8 more rats received placebo. After 24 hours, 3) diaphragm isometric contractile force was measured. The mRNA and protein levels of DHPRs and RyRs in diaphragm muscles were determined. Results : Sepsis weakened diaphragm contractile function. The expression levels of DHPRα1s and RyR1 were significantly lower in septic rats than in control rats. The expression levels of DHPRα1c and RyR3 were unaffected by sepsis. RyR2 was undetectable at both mRNA and protein levels in the control and sepsis groups. Conclusions : Weakened diaphragm contraction in the septic rats was associated with reduced mRNA and protein expression of DHPRα1s and RyR1, the isoforms of skeletal muscles. Muscle Nerve 48:745–751, 2013

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