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Bone marrow stem cells in facial nerve regeneration from isolated stumps
Author(s) -
Salomone Raquel,
Bento Ricardo F.,
Costa Heloisa J.Z.R.,
AzziNogueira Deborah,
Ovando Patrícia C.,
DaSilva Ciro F.,
Zanatta Daniela B.,
Strauss Bryan E.,
Haddad Luciana A.
Publication year - 2013
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.23768
Subject(s) - facial nerve , regeneration (biology) , silicone , anatomy , bone marrow , compound muscle action potential , schwann cell , histology , medicine , bone marrow stem cell , stem cell , pathology , chemistry , biology , electrophysiology , microbiology and biotechnology , organic chemistry
Severe lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting. Methods : We evaluated bone marrow stem cells ( BMSC ) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B–D, the tube was filled with acellular gel; and, in groups C and D, undifferentiated BMSC (uBMSC) or Schwann‐like cells differentiated from BMSC (dBMSC) were added, respectively. Compound muscle action potentials (CMAPs) were measured, and histology was evaluated. Results : Groups C and D had the highest CMAP amplitudes. Group C had shorter CMAP durations than groups A, B, and D. Distal axonal number and density were increased in group C compared with groups A and B. Conclusions : Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results. Muscle Nerve 48 : 423–429, 2013