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Growth‐associated protein‐43 expression in cocultures of dorsal root ganglion neurons and skeletal muscle cells with different neurotrophins
Author(s) -
Zhang Weiwei,
Miao Yajun,
Xing Ziying,
Li Hao,
Liu Huangxiang,
Li Zhenzhong
Publication year - 2013
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.23689
Subject(s) - dorsal root ganglion , neurotrophin , microbiology and biotechnology , biology , ganglion , skeletal muscle , dorsum , neurotrophin 3 , neuroscience , anatomy , neurotrophic factors , brain derived neurotrophic factor , biochemistry , receptor
Both target skeletal muscle (SKM) cells and neurotrophins (NTs) are essential for the maintenance of neuronal function and nerve–muscle communication. The effects of different NTs and SKM cells on growth‐associated protein‐43 (GAP‐43) expression in dorsal root ganglion (DRG) neurons have not been clarified. Methods: The morphological relationship between DRG neurons and SKM cells in neuromuscular cocultures was observed by scanning electron microscopy. The levels of GAP‐43 and its mRNA were determined after administration of different NTs. Results: DRG neurons demonstrated dense neurite outgrowth in the presence of NTs. Distinct NTs promoted GAP‐43 and its mRNA expression in neuromuscular cocultures of DRG neurons and SKM cells. Conclusions: These results offer new clues for a better understanding of the effects of distinct NTs on GAP‐43 expression in DRG sensory neurons in the presence of target SKM cells and implicate NTs and target SKM cells in DRG neuronal regeneration. Muscle Nerve 47: 909–915, 2013