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Homogenates of skeletal muscle injected with snake venom inhibit myogenic differentiation in cell culture
Author(s) -
SaraviaOtten Patricia,
Robledo BáRbara,
Escalante Teresa,
Bonilla Llira,
Rucavado Alexandra,
Lomonte Bruno,
Hernández Rosario,
Flock JanIngmar,
Gutiérrez José María,
Gastaldello Stefano
Publication year - 2013
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.23489
Subject(s) - venom , snake venom , myocyte , skeletal muscle , biology , necrosis , antibody , immunology , microbiology and biotechnology , medicine , endocrinology , biochemistry , genetics
Viperid snakebite envenomings are characterized by muscle necrosis and a deficient regenerative response. Methods: Homogenates from gastrocnemius muscles of mice injected with the venom of the snake Bothrops asper or with 2 tissue‐damaging toxins were added to cultures of C2C12 myogenic cells. Myoblasts proliferation and fusion were assessed. Venom was detected by immunoassay in mouse muscle during the first week after injection. Results: Homogenates from venom‐injected muscle induced a drop in the number of proliferating myoblasts and a complete elimination of myotube formation. The inhibitory effect induced by homogenates from venom‐injected mice was abrogated by preincubation of the homogenate with antivenom antibodies but not with control antibodies. This finding provides evidence that the effect is due to the action of venom in the tissue. Conclusions: Our observations suggest that traces of venom in muscle tissue might inhibit myotube formation and preclude a successful regenerative response. Muscle Nerve, 2013