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Myosin heavy chain isoform expression in human extraocular muscles: Longitudinal variation and patterns of expression in global and orbital layers
Author(s) -
Park KyungAh,
Lim Jeonghee,
Sohn Seongsoo,
Oh Sei Yeul
Publication year - 2012
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.23240
Subject(s) - gene isoform , extraocular muscles , myosin , biology , anatomy , microbiology and biotechnology , gene , genetics
: We investigated the distribution of myosin heavy chain (MyHC) isoforms along the length of the global and orbital layers of human extraocular muscles (EOMs). Methods : Whole muscle tissue extracts of human EOMs were cross‐sectioned consecutively and separated into orbital and global layers. The extracts from these layers were subjected to electrophoretic analysis, followed by quantification with scanning densitometry. Results : MyHC isoforms displayed different distributions along the lengths of EOMs. In the orbital and global layers of all EOMs except for the superior oblique muscle, MyHCeom was enriched in the central regions. MyHCIIa and MyHCI were most abundant in the proximal and distal ends. Conclusions : A variation in MyHC isoform expression was apparent along the lengths of human EOMs. These results provide a basis for understanding the molecular mechanisms underlying the functional diversity of EOMs. Muscle Nerve, 2012