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Using complementary DNA from MyoD‐transduced fibroblasts to sequence large muscle genes
Author(s) -
Waddell Leigh B.,
Monnier Nicole,
Cooper Sandra T.,
North Kathryn N.,
Clarke Nigel F.
Publication year - 2011
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.22118
Subject(s) - myod , nonsense mediated decay , gene , biology , complementary dna , messenger rna , genomic dna , microbiology and biotechnology , muscle biopsy , dna sequencing , dna , genetics , myogenesis , rna , rna splicing , medicine , biopsy , pathology
Large muscle genes are often sequenced using complementary DNA (cDNA) made from muscle messenger RNA (mRNA) to reduce the cost and workload associated with sequencing from genomic DNA. Two potential barriers are the availability of a frozen muscle biopsy, and difficulties in detecting nonsense mutations due to nonsense‐mediated mRNA decay (NMD). We present patient examples showing that use of MyoD‐transduced fibroblasts as a source of muscle‐specific mRNA overcomes these potential difficulties in sequencing large muscle–related genes. Muscle Nerve, 2011