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Axon cytoskeleton ultrastructure in chronic inflammatory demyelinating polyneuropathy
Author(s) -
Fressinaud Catherine,
Dubas Frédéric
Publication year - 2011
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.22069
Subject(s) - immunolabeling , chronic inflammatory demyelinating polyneuropathy , axon , ultrastructure , neurofilament , myelin , pathology , intermediate filament , cytoskeleton , schwann cell , nerve fiber , biology , brainstem , medicine , chemistry , anatomy , neuroscience , cell , immunology , central nervous system , immunohistochemistry , biochemistry , antibody
To detail the extent and pattern of axon cytoskeleton alterations in chronic inflammatory demyelinating polyneuropathy (CIDP).Methods: Nerve biopsies from 7 cases of CIDP, including 4 cases with severe fiber loss, were compared with 5 controls by morphometric transmission electron microscopy (TEM).Results: Despite demyelination of single fibers, myelin ultrastructure was otherwise normal. Contrary to immunolabeling, TEM revealed a decrease in neurofilament (NF) density in every case, although there were pronounced variations among fibers even in the same sample. The NF decrease reached the same extent in large‐ and small‐diameter fibers. It was observed in normally myelinated fibers, suggesting they were demyelinated at a distance from the section. Minimal inter‐NF distance increased roughly inversely to NF density. Microtubules increased in 3 cases previously characterized by increased growth‐associated protein (GAP‐43) immunolabeling.Conclusion:These data demonstrate the severity and constancy of axonal lesions, and especially of NF, in residual fibers in our cases of CIDP. Muscle Nerve 44: 332–339, 2011