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Patterns of gene expression in muscle and fat in tumor‐bearing rats: Effects of CRF2R agonist on cachexia
Author(s) -
Argilés Josep M.,
FontesOliveira Cibely Cristine,
Fuster Gemma,
Ametller Elisabet,
Figueras Maite,
Olivan Mireia,
LopezSoriano Francisco J.,
Qu Xiaoyan,
Demuth Jeffrey,
Stevens Paula,
Varbanov Alex,
Wang Feng,
Isfort Robert J.,
Busquets Sílvia
Publication year - 2010
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21781
Subject(s) - agonist , adipose tissue , endocrinology , cachexia , white adipose tissue , medicine , gene expression , biology , skeletal muscle , receptor , muscle tissue , gene , cancer , biochemistry
The hypothesis we tested was that administering corticotropin‐releasing factor receptor agonists preserves muscle mass during cancer that is related to changes in tissue gene expression. cDNA microarrays were used to compare mRNAs from muscle and adipose tissues of non‐treated and agonist‐treated tumor‐bearing rats. In muscle of non–tumor‐bearing agonist‐treated animals we observed decreased expression of genes associated with fatty acid uptake and esterification. In tumor‐bearing animals, CRF2R agonist administration produced decreased mRNA content of the atrogene lipin‐1. In white adipose tissue, agonist treatment of non–tumor‐bearing animals induced genes typically related to muscle structure and function. The fact that this treatment decreased expression of atrogenes could have clinical application. In addition, agonist treatment changed the gene pattern of adipose tissue to render it similar to that of skeletal muscle; thus, treatment with this agonist alters the gene pattern to what could be called “muscularization of white adipose tissue.” Muscle Nerve 42:936–949, 2010