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Sequencing protocols to genotype mdx , mdx 4cv , and mdx 5cv mice
Author(s) -
Banks Glen B.,
Combs Ariana C.,
Chamberlain Jeffrey S.
Publication year - 2010
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21700
Subject(s) - mdx mouse , dystrophin , duchenne muscular dystrophy , genotyping , point mutation , false positive paradox , genetics , polymerase chain reaction , biology , mutant , genotype , microbiology and biotechnology , gene , computer science , artificial intelligence
Abstract Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx , mdx 4cv , and mdx 5cv mice. This method clearly distinguishes between wildtype, heterozygous, and mutant transcripts, and thereby time and money can be saved by avoiding false positives. Muscle Nerve, 2010