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Neuromuscular junction morphology, fiber‐type proportions, and satellite‐cell proliferation rates are altered in MyoD −/− mice
Author(s) -
Macharia Raymond,
Otto Anthony,
Valasek Petr,
Patel Ketan
Publication year - 2010
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21637
Subject(s) - myod , biology , skeletal muscle , myogenesis , microbiology and biotechnology , wnt signaling pathway , myocyte , medicine , endocrinology , signal transduction
Abstract Gene compensation by members of the myogenic regulatory factor (MRF) family has been proposed to explain the apparent normal adult phenotype of MyoD −/− mice. Nerve and field stimulation were used to investigate contraction properties of muscle from MyoD −/− mice, and molecular approaches were used to investigate satellite‐cell behavior. We demonstrate that MyoD deletion results in major alterations in the organization of the neuromuscular junction, which have a dramatic influence on the physiological contractile properties of skeletal muscle. Second, we show that the lineage progression of satellite cells (especially initial proliferation) in the absence of MyoD is abnormal and linked to perturbations in the nuclear localization of β‐catenin, a key readout of canonical Wnt signaling. These results show that MyoD has unique functions in both developing and adult skeletal muscle that are not carried out by other members of the MRF family. Muscle Nerve, 2010