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Induction of myogenic differentiation by SDF‐1 via CXCR4 and CXCR7 receptors
Author(s) -
Melchionna Roberta,
Di Carlo Anna,
De Mori Roberta,
Cappuzzello Claudia,
Barberi Laura,
Musarò Antonio,
Cencioni Chiara,
Fujii Nobutaka,
Tamamura Hirokazu,
Crescenzi Marco,
Capogrossi Maurizio C.,
Napolitano Monica,
Germani Antonia
Publication year - 2010
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21611
Subject(s) - myogenesis , c2c12 , microbiology and biotechnology , myocyte , cellular differentiation , cxc chemokine receptors , stromal cell , biology , cxcr4 , receptor , chemistry , chemokine receptor , cancer research , chemokine , biochemistry , gene
The stromal cell–derived factor (SDF)‐1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF‐1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and real‐time polymerase chain reaction (PCR) were performed to evaluate in vitro the effect of SDF‐1 and CXCR4 and CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, and SDF‐1; during myogenic differentiation, CXCR4 and CXCR7 levels are downregulated, and SDF‐1 release is decreased. SDF‐1 anticipates myosin heavy chain accumulation and myotube formation in both C2C12 myoblasts and satellite cells. Interestingly, inhibition of CXCR4 and CXCR7 signaling, either by drugs or RNA interfererence, blocks myogenic differentiation. Further, the CXCR4 antagonist, 4F‐benzoyl‐TN14003, inhibits myoblast cell cycle withdrawal and decreases the retinoblastoma gene (pRb) product accumulation in its hypophosphorylated form. Our experiments demonstrate that SDF‐1 regulates myogenic differentiation via both CXCR4 and CXCR7 chemokine receptors. Muscle Nerve 000:000–000, 2010