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Measurement of platelet‐derived microparticle levels using an enzyme‐linked immunosorbent assay in polymyositis and dermatomyositis patients
Author(s) -
Shirafuji Toshihiko,
Hamaguchi Hirotoshi,
Higuchi Masatsugu,
Kanda Fumio
Publication year - 2009
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21311
Subject(s) - polymyositis , dermatomyositis , interquartile range , platelet , medicine , glucocorticoid , endocrinology , platelet activation , chemistry , immunology , gastroenterology
Platelet‐derived microparticle (PDMP) levels were measured using an enzyme‐linked immunosorbent assay (ELISA) to elucidate the role of platelet activation in patients with polymyositis or dermatomyositis (PM/DM). PDMP levels in active PM/DM patients (median 13.3 U/ml, interquartile range 9.9–20.7 U/ml, n = 16) and those in patients undergoing treatment (12.1 U/ml, 7.4–16.7 U/ml, n = 12) were significantly higher than in controls (6.5 U/ml, 5.0–8.4 U/ml, n = 26, vs. active, P = 0.0001; vs. treatment, P = 0.004). In a paired sampling study, PDMP decreased significantly after glucocorticoid treatment ( P = 0.04). PDMP in the active PM/DM patients correlated significantly with serum C‐reactive protein levels ( r s = 0.67, P = 0.01). These results suggest that platelets may play an important role in the inflammatory process, and that PDMP level could be a useful marker of inflammatory activity in PM/DM patients. Muscle Nerve 39: 586–590, 2009