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Decreased muscle atrophy F‐box (MAFbx) expression in regenerating muscle after muscle‐damaging exercise
Author(s) -
Okada Atsushi,
Ono Yusuke,
Nagatomi Ryoichi,
Kishimoto Koshi N.,
Itoi Eiji
Publication year - 2008
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21110
Subject(s) - myod , muscle atrophy , downregulation and upregulation , myod protein , myosin , skeletal muscle , soleus muscle , endocrinology , medicine , gastrocnemius muscle , myocyte , protein degradation , atrophy , biology , anatomy , chemistry , myogenesis , microbiology and biotechnology , biochemistry , gene
A muscle‐specific ubiquitin ligase, Muscle Atrophy F‐box (MAFbx), is known to mediate the degradation of muscle‐specific transcription factor MyoD in vitro. Its regulation in regenerating skeletal muscle, however, has not been clarified. We looked for evidence of MAFbx downregulation in the course of regeneration after muscle damaging exercise. The soleus and gastrocnemius muscles of mice were subjected to forced eccentric contraction by electrical stimulation to induce muscle damage. The expression of developmental myosin heavy chain (MHCd) suggested that muscle regeneration took place from Day 3 after exercise. mRNA and protein expression of MAFbx decreased on Days 3, 5, and 7, while MyoD protein increased on Days 3, 5, and 7. Although further study is required to establish the causal relationships, downregulation of MAFbx may have reduced MyoD degradation in favor of muscle regeneration. Muscle Nerve, 2008