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Analysis of the benefits of vitamin cocktails in treating Charcot–Marie–Tooth disease type 1A
Author(s) -
Kaya Ferdinand,
Belin Sophie,
Micallef Joelle,
Blin Olivier,
Fontés Michel
Publication year - 2008
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21071
Subject(s) - ascorbic acid , transfection , vitamin c , schwann cell , vitamin , cell , medicine , chemistry , biochemistry , pathology , gene , food science
We recently proposed that the use of high doses of ascorbic acid (AA) could constitute the first potential treatment for Charcot–Marie–Tooth disease type 1A (CMT1A).4 We investigated the potential benefits of using cocktails of vitamins for CMT1A therapy. We used transient transfection of Schwann cells with a construction placing the expression of a reporter gene under the control of the Schwann cell‐specific promoter of PMP22 . Transfected cells were cultured with or without addition of ascorbic acid, vitamin A, vitamin E, or a cocktail of these vitamins. Adding vitamin A or E counteracts the effect of ascorbic acid in inhibiting PMP22 expression. We thus recommend that vitamins A and E should not be included in combination with AA in clinical trials. Muscle Nerve, 2008