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PAX3/7 expression coincides with myod during chronic skeletal muscle overload
Author(s) -
Hyatt JonPhilippe K.,
McCall Gary E.,
Kander Elizabeth M.,
Zhong Hui,
Roy Roland R.,
Huey Kimberly A.
Publication year - 2008
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.21006
Subject(s) - myod , skeletal muscle , pax3 , medicine , physical medicine and rehabilitation , biology , genetics , myogenesis , transcription factor , gene
Paired box (Pax) proteins 3 and 7 are key determinants for embryonic skeletal muscle development by initiating myogenic regulatory factor (MRF) gene expression. We show that Pax3 and 7 participate in adult skeletal muscle plasticity during the initial responses to chronic overload (≤7 days) and appear to coordinate MyoD expression, a member of the MRF family of genes. Pax3 and 7 mRNA were higher than control within 12 h after initiation of overload, preceded the increase in MyoD mRNA on day 1, and peaked on day 2. On days 3 and 7, Pax7 mRNA remained higher than control, suggesting that satellite cell self‐renewal was occurring. Pax3 and 7 and MyoD protein levels were higher than control on days 2 and 3. These data indicate that Pax3 and 7 coordinate the recapitulation of developmental‐like regulatory mechanisms in response to growth‐inducing stimuli in adult skeletal muscle, presumably through activation of satellite cells. Muscle Nerve, 2008