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Expression of caveolin‐3 immunoreactivities in the developing sciatic nerve of the rat
Author(s) -
Lee Ho J.,
Park Choong H.,
Lee Seong J.,
Park Jin W.,
Choi Jong H.,
Ryu Gi H.,
Kwon Bum S.
Publication year - 2008
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20973
Subject(s) - sciatic nerve , caveolin 1 , medicine , neuroscience , caveolae , endocrinology , anatomy , biology , microbiology and biotechnology , signal transduction
Caveolae are formed by the caveolin (CAV) family of proteins, CAV‐1, ‐2, and ‐3. CAV‐1 and ‐2 are co‐expressed in many cell types, whereas CAV‐3 is muscle‐specific and mutation of the CAV‐3 gene causes muscular dystrophy. CAV‐3 has also been detected in brain astroglial cells and in peripheral nerves along with CAV‐1. Therefore, we sought to determine whether CAV‐3 protein is expressed in developing peripheral nerves by using immunohistochemistry and reverse transcription–polymerase chain reaction (RT‐PCR). We found that CAV‐3 immunoreactivities (IRs) were localized in the myelin sheath during peripheral nerve development. CAV‐3 IRs were intense during the early postnatal stage, but decreased as the peripheral nerves matured at postnatal weeks 3–5. CAV‐3 mRNA expression was also markedly decreased during postnatal development. Because the expression pattern of CAV‐3 IRs was opposite that of CAV‐1 IRs, CAV‐1 and ‐3 might be involved in different phases of peripheral nerve myelination and play complementary roles in myelin maturation and peripheral nerve development. © 2008 Wiley Periodicals, Inc. Muscle Nerve 2008