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Fluorophore‐labeled myosin‐specific antibodies simplify muscle‐fiber phenotyping
Author(s) -
Gregorevic Paul,
Meznarich Norman A.,
Blankinship Michael J.,
Crawford Robert W.,
Chamberlain Jeffrey S.
Publication year - 2008
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20877
Subject(s) - myosin , skeletal muscle , fluorophore , gene isoform , myocyte , biology , microbiology and biotechnology , biochemistry , anatomy , fluorescence , physics , quantum mechanics , gene
Skeletal muscles are frequently analyzed for composition of phenotypically distinct myofibers, as a functional determinant. We describe an improved myofiber phenotyping procedure, involving cryosection co‐incubation with fluorophore‐labeled myosin heavy‐chain (MyHC)‐isoform–specific antibodies. This technique identifies multiple fiber “types” on a single section, thereby reducing reagents and processing, and offers side‐by‐side comparison of samples from multiple species including mice. These advances are valuable for studying the physiological attributes of skeletal muscle in health and disease. Muscle Nerve, 2007