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Catalase overexpression does not impair extensor digitorum longus muscle function in normal mice
Author(s) -
Liu Mingju,
Yue Yongping,
Li Dejia,
Duan Dongsheng
Publication year - 2007
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20874
Subject(s) - catalase , extensor digitorum longus muscle , western blot , adeno associated virus , biology , enzyme , muscle contraction , immunostaining , skeletal muscle , endocrinology , medicine , chemistry , biochemistry , immunohistochemistry , immunology , vector (molecular biology) , gene , recombinant dna
Catalase is a major antioxidant enzyme. Increasing catalase expression represents a promising avenue to improve muscle function in certain physiological conditions and in some muscle diseases. We hypothesized that catalase overexpression should not impair normal muscle contraction. We delivered a hemagglutinin (HA)‐tagged human catalase gene to normal mouse muscle by an adeno‐associated viral vector (AAV). Western blot and immunostaining revealed efficient expression of HA‐tagged catalase. Enzymatic assay demonstrated an approximately threefold increase in catalase activity in AAV‐infected muscles. Catalase overexpression impaired neither twitch nor tetanic tension in the extensor digitorum longus (EDL) muscle. Furthermore, EDL fatigue response was not altered. Taken together, we have developed a novel AAV vector to enhance catalase expression. Lack of apparent toxicity in normal muscle strongly supports further exploration of this vector to reduce oxidative stress–induced muscle damage. Muscle Nerve, 2007