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Electrical stimulation based on chronaxie reduces atrogin‐1 and myoD gene expressions in denervated rat muscle
Author(s) -
Russo Thiago Luiz,
Peviani Sabrina Messa,
Freria Camila Marques,
GigoBenato Davilene,
Geuna Stefano,
Salvini Tania Fátima
Publication year - 2007
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20668
Subject(s) - myod , denervation , skeletal muscle , stimulation , muscle atrophy , atrophy , medicine , endocrinology , myogenesis
Denervation induces muscle fiber atrophy and changes in the gene expression rates of skeletal muscle. Electrical stimulation (ES) is a procedure generally used to treat denervated muscles in humans. This study evaluated the effect of ES based on chronaxie and rheobase on the expression of the myoD and atrogin‐1 genes in denervated tibialis anterior (TA) muscle of Wistar rats. Five groups were examined: (1) denervated (D); (2) D+ES; (3) sham denervation; (4) normal (N); and (5) N+ES. Twenty muscle contractions were stimulated every 48 h using surface electrodes. After 28 days, ES significantly decreased the expression of myoD and atrogin‐1 in D+ES compared to the D group. However, ES did not prevent muscle‐fiber atrophy after denervation. Thus, ES based on chronaxie values and applied to denervated muscles using surface electrodes, as normally used in human rehabilitation, was able to reduce the myoD and atrogin‐1 gene expressions, which are related to muscular growth and atrophy, respectively. The results of this study provide new information for the treatment of denervated skeletal muscle using surface ES. Muscle Nerve, 2006