z-logo
Premium
Molecular and cellular defects of skeletal muscle in an animal model of acute quadriplegic myopathy
Author(s) -
Mozaffar Tahseen,
Haddad Fadia,
Zeng Ming,
Zhang Li Ying,
Adams Greg R.,
Baldwin Kenneth M.
Publication year - 2007
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20647
Subject(s) - denervation , myosin , endocrinology , medicine , muscle atrophy , myopathy , atrophy , glucocorticoid , dexamethasone , biology , skeletal muscle , messenger rna , microbiology and biotechnology , gene , biochemistry
Muscle denervation and concomitant high‐dose dexamethasone treatment in rodents produces characteristic pathologic features of severe muscle atrophy and selective myosin heavy filament (MyHC) depletion, identical to those seen in acute quadriplegic myopathy (AQM), also known as critical illness myopathy. We tested the hypothesis that defective pre‐translational processes contribute to the atrophy and selective MyHC depletion in this model. We examined the effects of combined glucocorticoid–denervation treatment on MyHC and actin mRNA populations; we also studied mRNA expression of the myogenic regulatory factors (MRFs), primary transcription factors for MyHC. Adult female rats were subjected to proximal sciatic denervation followed by high‐dose dexamethasone (DD) treatment (5 mg/kg body weight daily) for 7 days. Disease controls included rats treated with denervation alone (DN) or dexamethasone alone (DX). At 1 week the plantaris atrophied by ∼42% in DD muscles. DD treatment resulted in selective MyHC protein depletion; actin protein concentration was not significantly changed. Despite an increase in total RNA concentration in DN and DD muscles, MyHC and actin mRNA concentrations were significantly decreased in these muscles. MyHC mRNA showed a significantly more extensive depletion relative to actin mRNA in DD muscles. Glucocorticoid treatment did not influence a denervation‐induced increase in the mRNA expression of the MRFs. We conclude that a deleterious interaction between glucocorticoid and denervation treatments in skeletal muscle is responsible for pre‐translational defects that reduce actin and MyHC mRNA substrates in a disproportionate fashion. The resultant selective MyHC depletion contributes to the severe muscle atrophy. Muscle Nerve, 2006

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here