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CK‐MM autoantibodies: Prevalence, immune complexes, and effect on CK clearance
Author(s) -
Warren Gordon L.,
O'Farrell Laura,
Rogers Karen R.,
Billings Kelly M.,
Sayers Stephen P.,
Clarkson Priscilla M.
Publication year - 2006
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20594
Subject(s) - autoantibody , creatine kinase , clearance rate , medicine , endocrinology , antibody , immune system , immunology
Although the blood level of creatine kinase (CK) is the most commonly used marker of muscle injury, there is marked interindividual variability in this measure. Part of this variability may be attributed to variability in the rate of CK clearance from the circulation. In this study, we assessed the possibility that CK‐MM autoantibodies form immune complexes with CK following muscle injury and subsequently affect the CK clearance rate. Using an enzyme‐linked immunosorbent assay, CK‐MM autoantibodies were detected in all 25 human subjects studied but the levels varied greatly. Using protein A‐sepharose, the percentage of the plasma CK activity found in immune complexes was determined to be correlated with the CK‐MM autoantibody level at lower CK levels (<1,022 U/L). When CK‐MM antibodies were administered to mice, plasma CK activity following a bolus CK injection was reduced by 11%–32%. We conclude that CK‐MM autoantibodies can modulate the rate of CK clearance from the circulation. Thus, the relatively low blood CK levels seen in some individuals following injury may be attributed partly or entirely to an autoantibody‐enhanced clearance of CK. Muscle Nerve, 2006