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Short‐ or long‐term effects of adult myoblast transfer on properties of reinnervated skeletal muscles
Author(s) -
Coulet Bertrand,
Lacombe Fabien,
Lazerges Cyril,
Daussin PaulAndré,
Rossano Bernadette,
Micallef JeanPaul,
Chammas Michel,
Reyne Yves,
Bacou Francis
Publication year - 2006
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20459
Subject(s) - reinnervation , medicine , skeletal muscle , myocyte , anatomy , denervation , common peroneal nerve
Skeletal muscle demonstrates a force deficit after repair of injured peripheral nerves. Data from the literature indicate that myoblast transfer enhances recovery of muscle function. Thus, we tested the hypothesis that transfer of adult myoblasts improves the properties of reinnervated rabbit tibialis anterior (TA) muscles in both the short term (4 months) and long term (14 months). Two months after transection and immediate suture of the common peroneal nerve, TA muscles were made to degenerate by cardiotoxin injection and then transplanted with adult myoblasts cultured for 13 days. Under these conditions, muscles studied at 4 months were heavier, contained larger fibers, and developed a significantly higher maximal force than muscles that had only been denervated–reinnervated. In the long term, although muscles made to degenerate were heavier and developed a significantly higher maximal force than denervated–reinnervated muscles, myoblast transfer failed to improve these parameters. However, the overall characteristics of long‐term operated muscles tended clearly to approach those of the controls. Taken together, these results may have significant implications in certain orthopedic contexts, particularly after immediate or delayed muscle reinnervation. Muscle Nerve, 2006