Premium
Effects of exercise and steroid on skeletal muscle apoptosis in the mdx mouse
Author(s) -
Lim JeongHoon,
Kim DaiYoul,
Bang Moon Suk
Publication year - 2004
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.20139
Subject(s) - duchenne muscular dystrophy , tunel assay , apoptosis , dna fragmentation , skeletal muscle , endocrinology , mdx mouse , medicine , muscular dystrophy , steroid , hindlimb , biology , immunohistochemistry , dystrophin , programmed cell death , biochemistry , hormone
Reports concerning the influence of exercise loading and steroid administration on dystrophinopathy are inconsistent. To investigate the effect of muscle exercise in Duchenne muscular dystrophy (DMD), 15 control and 15 mdx mice, an animal model of DMD, were divided into free‐living ( n = 6), exercise ( n = 6), and immobilization ( n = 3) groups. Free‐living and exercise groups were further divided into steroid‐treated and sham‐treated groups to evaluate the effect of steroid administration. We measured apoptotic changes by in situ DNA nick‐end labeling (TUNEL), DNA fragmentation assay, and Western blotting for Bcl‐2 and BAX. Apoptosis was most prominent in the sham‐treated exercise group, and it was significantly reduced in the steroid‐treated exercise group. The steroid‐treated free‐living group showed a higher rate of apoptotic change than the sham‐treated free‐living group. Apoptosis was minimized in the free‐living condition, whereas exercise loading and immobilization caused apoptotic change in this muscular dystrophy animal model. Steroid administration induced apoptosis in muscle of free‐living mice, but alleviated the apoptotic damage caused by exercise loading in mdx mice. These findings suggest that steroid administration may be effective in preventing a postexercise deterioration of skeletal muscle in animal models of DMD. Muscle Nerve 30: 456–462 2004