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High efficiency transfection of primary skeletal muscle cells with lipid‐based reagents
Author(s) -
Neuhuber Birgit,
Huang David I.,
Daniels Mathew P.,
Torgan Carol E.
Publication year - 2002
Publication title -
muscle and nerve
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 145
eISSN - 1097-4598
pISSN - 0148-639X
DOI - 10.1002/mus.10171
Subject(s) - transfection , myogenesis , skeletal muscle , myocyte , primary cell , cell culture , microbiology and biotechnology , biology , chemistry , cell , biochemistry , anatomy , genetics
Abstract Lipofection is a convenient method for gene transfer into muscle cells but reportedly is inefficient. We tested the efficacy of commercially available lipid‐based and polyamine transfection reagents. Primary rat skeletal muscle cell cultures were transfected at three stages of development and assayed after fusion. Efficiency reached 30% during the proliferation stage and up to 23% when most myoblasts had fused into myotubes. Optimization of transfection conditions with three different vectors yielded efficiencies exceeding 50%. Thus, lipid‐based transfection into primary skeletal muscle cells can be several times more efficient than previously reported. © 2002 Wiley Periodicals, Inc. Muscle Nerve 26: 136–140, 2002