Open AccessPeriodic mRNA synthesis and degradation co‐operate during cell cycle gene expression
Author(s) -
Eser Philipp,
Demel Carina,
Maier Kerstin C,
Schwalb Björn,
Pirkl Nicole,
Martin Dietmar E,
Cramer Patrick,
Tresch Achim
Publication year - 2014
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.1002/msb.134886
Subject(s) - biology , messenger rna , cell cycle , gene expression , transcriptome , microbiology and biotechnology , transcription (linguistics) , gene , p bodies , rna , translation (biology) , biochemistry , linguistics , philosophy
During the cell cycle, the levels of hundreds of mRNA s change in a periodic manner, but how this is achieved by alterations in the rates of mRNA synthesis and degradation has not been studied systematically. Here, we used metabolic RNA labeling and comparative dynamic transcriptome analysis ( cDTA ) to derive mRNA synthesis and degradation rates every 5 min during three cell cycle periods of the yeast S accharomyces cerevisiae . A novel statistical model identified 479 genes that show periodic changes in mRNA synthesis and generally also periodic changes in their mRNA degradation rates. Peaks of mRNA degradation generally follow peaks of mRNA synthesis, resulting in sharp and high peaks of mRNA levels at defined times during the cell cycle. Whereas the timing of mRNA synthesis is set by upstream DNA motifs and their associated transcription factors ( TF s), the synthesis rate of a periodically expressed gene is apparently set by its core promoter.