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Magnetic resonance and diffusion tensor imaging of the adolescent rabbit growth plate of the knee
Author(s) -
Kvist Ola,
Damberg Peter,
Dou Zelong,
Berglund Johan Sanmartin,
Flodmark CarlErik,
Nilsson Ola,
Diaz Sandra
Publication year - 2023
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.29432
Subject(s) - fractional anisotropy , diffusion mri , magnetic resonance imaging , intraclass correlation , anatomy , medicine , nuclear medicine , effective diffusion coefficient , tibia , femur , radiology , surgery , clinical psychology , psychometrics
Purpose To assess the ability of MRI‐DTI to evaluate growth plate morphology and activity compared with that of histomorphometry and micro‐CT in rabbits. Methods The hind limbs of female rabbits aged 16, 20, and 24 wk (n = 4 per age group) were studied using a 9.4T MRI scanner with a multi‐gradient echo 3D sequence and DTI in 14 directions ( b ‐value = 984 s/mm 2 ). After MRI, the right and left hind limb were processed for histological analysis and micro‐CT, respectively. The Wilcoxon signed‐rank test was used to evaluate the height and volume of the growth plate. Intraclass correlation and Pearson correlation coefficient were used to evaluate the association between DTI metrics and age. Results The growth plate height and volume were similar for all modalities at each time point and age. Age was correlated with all tractography and DTI metrics in both the femur and tibia. A correlation was also observed between all the metrics at both sites. Tract number and volume declined with age; however, tract length did not show any changes. The fractional anisotropy color map showed lateral diffusion centrally in the growth plate and perpendicular diffusion in the hypertrophic zone, as verified by histology and micro‐CT. Conclusion MRI‐DTI may be useful for evaluating the growth plates.