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NMR visibility of deuterium‐labeled liver glycogen in vivo
Author(s) -
De Feyter Henk M.,
Thomas Monique A.,
Behar Kevin L.,
Graaf Robin A.
Publication year - 2021
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.28717
Subject(s) - glycogen , in vivo , chemistry , glycogen branching enzyme , glycogen synthase , glycogenesis , nuclear magnetic resonance spectroscopy , deuterium , biochemistry , biology , stereochemistry , microbiology and biotechnology , physics , quantum mechanics
Purpose Deuterium metabolic imaging (DMI) combined with [6,6’‐ 2 H 2 ]‐glucose has the potential to detect glycogen synthesis in the liver. However, the similar chemical shifts of [6,6’‐ 2 H 2 ]‐glucose and [6,6’‐ 2 H 2 ]‐glycogen in the 2 H NMR spectrum make unambiguous detection and separation difficult in vivo, in contrast to comparable approaches using 13 C MRS. Here the NMR visibility of 2 H‐labeled glycogen is investigated to better understand its potential contribution to the observed signal in liver following administration of [6,6’‐ 2 H 2 ]‐glucose. Methods Mice were provided drinking water containing 2 H‐labeled glucose. High‐resolution NMR analyses was performed of isolated liver glycogen in solution, before and after the addition of the glucose‐releasing enzyme amyloglucosidase. Results 2 H‐labeled glycogen was barely detectable in solution using 2 H NMR because of the very short T 2 (<2 ms) of 2 H‐labeled glycogen, giving a spectral line width that is more than five times as broad as that of 13 C‐labeled glycogen (T 2 = ~10 ms). Conclusion 2 H‐labeled glycogen is not detectable with 2 H MRS(I) under in vivo conditions, leaving 13 C MRS as the preferred technique for in vivo detection of glycogen.