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Bulk volume susceptibility difference between deoxyhemoglobin and oxyhemoglobin for HbA and HbS: A comparative study
Author(s) -
Eldeniz Cihat,
Binkley Michael M.,
Fields Melanie,
Guilliams Kristin,
Ragan Dustin K.,
Chen Yasheng,
Lee JinMoo,
Ford Andria L.,
An Hongyu
Publication year - 2021
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.28668
Subject(s) - hemoglobin , sickle cell anemia , fetal hemoglobin , hemoglobin f , medicine , anemia , deoxygenated hemoglobin , chemistry , venous blood , nuclear magnetic resonance , cardiology , pregnancy , fetus , biology , physics , disease , genetics
Purpose Sickle cell anemia is a blood disorder that alters the morphology and the oxygen affinity of the red blood cells. Cerebral oxygen extraction fraction measurements using quantitative BOLD contrast have been used for assessing inadequate oxygen delivery and the subsequent risk of ischemic stroke in sickle cell anemia. The BOLD signal in MRI studies relies on Δ χ do , the bulk volume susceptibility difference between fully oxygenated and fully deoxygenated blood. Several studies have measured Δ χ dofor normal hemoglobin A (HbA). However, it is not known whether the value is different for sickle hemoglobin. In this study, Δ χ dowas measured for both HbA and sickle hemoglobin. Methods Six sickle cell anemia patients and 6 controls were recruited. Various blood oxygenation levels were achieved through in vivo manipulations to keep the blood close to its natural state. To account for the differences in oxygen affinity, Hill’s equations were used to translate partial pressure of oxygen to oxygen saturation for HbA, sickle hemoglobin, and fetal hemoglobin (HbF) separately. The pH and PCO 2 corrections were performed. Temperature and magnetic field drift were controlled for. A multivariate generalized linear mixed model with random participant effect was used. Results Assuming that Δ χ dois similar for HbA and HbF and that Δ χ metHbis 5/4 of Δ χ dofor HbA, it was found that the Δ χ dovalues for HbA and sickle hemoglobin were not statistically significantly different from each other. Conclusion The same Δ χ dovalue can be used for both types of hemoglobin in quantitative BOLD analysis.