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Increasing the sensitivity of hyperpolarized [ 15 N 2 ]urea detection by serial transfer of polarization to spin‐coupled protons
Author(s) -
Kreis Felix,
Wright Alan J.,
Somai Vencel,
KatzBrull Rachel,
Brindle Kevin M.
Publication year - 2020
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.28241
Subject(s) - hyperpolarization (physics) , polarization (electrochemistry) , pulse sequence , chemistry , nuclear magnetic resonance , proton , analytical chemistry (journal) , atomic physics , molecular physics , nuclear magnetic resonance spectroscopy , physics , chromatography , quantum mechanics
Purpose Hyperpolarized 15 N‐labeled molecules have been proposed as imaging agents for investigating tissue perfusion and pH. However, the sensitivity of direct 15 N detection is limited by the isotope's low gyromagnetic ratio. Sensitivity can be increased by transferring 15 N hyperpolarization to spin‐coupled protons provided that there is not significant polarization loss during transfer. However, complete polarization transfer would limit the temporal window for imaging to the order of the proton T 1 (2‐3 s). To exploit the long T 1 offered by storing polarization in 15 N and the higher sensitivity of 1 H detection, we have developed a pulse sequence for partial polarization transfer. Methods A polarization transfer pulse sequence was modified to allow partial polarization transfer, as is required for dynamic measurements, and that can be implemented with inhomogeneous B 1 fields, as is often the case in vivo. The sequence was demonstrated with dynamic spectroscopy and imaging measurements with [ 15 N 2 ]urea. Results When compared to direct 15 N detection, the sequence increased the signal‐to‐noise ratio (SNR) by a factor of 1.72 ± 0.25, where both experiments depleted ~20% of the hyperpolarization (>10‐fold when 100% of the hyperpolarization is used). Simulations with measured cross relaxation rates showed that this sequence gave up to a 50‐fold increase in urea proton polarization when compared to spontaneous polarization transfer via cross relaxation. Conclusion The sequence gave an SNR increase that was close to the theoretical limit and can give a significant SNR benefit when compared to direct 13 C detection of hyperpolarized [ 13 C]urea.