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Use of low field nuclear magnetic resonance to monitor lung inflammation and the amount of pathological components in the sputum of cystic fibrosis patients
Author(s) -
Abrami Michela,
Maschio Massimo,
Conese Massimo,
Confalonieri Marco,
Di Gioia Sante,
Gerin Fabio,
Dapas Barbara,
To Federica,
Farra Rossella,
Murano Erminio,
Zanella Giada,
Salton Francesco,
Torelli Lucio,
Grassi Gabriele,
Grassi Mario
Publication year - 2020
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.28115
Subject(s) - sputum , medicine , inflammation , cystic fibrosis , lung , fibrosis , magnetic resonance imaging , tumor necrosis factor alpha , gastroenterology , pathology , immunology , radiology , tuberculosis
Purpose To develop a novel approach to monitor lung ventilation/inflammation in cystic fibrosis (CF) patients. Lung assessment in CF patients is relevant given that most patients succumb to respiratory failure. Respiratory functional tests (forced expiratory volume in the first second; FEV 1 ) and inflammatory markers are used to test pulmonary ventilation/inflammation, respectively. However, FEV 1 is effort dependent and might be uncomfortable for CF patients. Furthermore, inflammatory marker detection is costly and not rapid. To overcome these limitations, we propose the measurement, by means of low field nuclear magnetic resonance, of the spin‐spin relaxation time ( T 2m ) of water hydrogens present in CF patient sputum. In CF sputum, different biological components are pathologically increased and inversely related to lung functionality. Moreover, we showed that these components alter in a dose‐dependent manner the T 2m in synthetic CF sputum. Methods Sputum samples were obtained from 42 CF subjects by voluntary expectoration; FEV 1 , C‐reactive protein (CRP), blood neutrophil counts together with cytokine (tumor necrosis factor alpha [TNFα], interleukin [IL]‐1β, IL‐4, and vascular endothelial growth factor) quantifications were then evaluated. Results In sputum samples, we observe that T 2m directly correlates ( r FEV1 = 0.44; P < 10 −4 ; 169 samples) with FEV 1 . Moreover, T 2m inversely correlates with the circulating inflammation markers CRP/neutrophil number ( r CRP = −0.44, P < 10 −4 ; r NC = −0.37, P < 2 * 10 −4 ; 103 and 86 samples, respectively) and with the sputum inflammatory cytokines TNFα/IL‐β1 ( r TNFα = −0.72, P < 10 −4 ; r IL‐1β = −0.685, P < 10 −4 ; 27 samples). T 2m variations also correspond to FEV 1 values over time in defined patients. Conclusion These findings, together with the fast, reliable, and simple determination of T 2m , make our approach a novel tool potentially usable in the real world of CF patients.