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An efficient MRI agent targeting extracellular markers in prostate adenocarcinoma
Author(s) -
Pagoto Amerigo,
Tripepi Martina,
Stefania Rachele,
Lanzardo Stefania,
Livio Longo Dario,
Garello Francesca,
Porpiglia Francesco,
Manfredi Matteo,
Aime Silvio,
Terreno Enzo
Publication year - 2019
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.27494
Subject(s) - in vivo , chemistry , gadolinium , prostate cancer , peptide , prostate , in vitro , nuclear magnetic resonance , pathology , microbiology and biotechnology , cancer , medicine , biochemistry , biology , physics , organic chemistry
Purpose Prostate cancer (PCa) is the most widespread tumor affecting males in Western countries. We propose a novel MRI molecular tetrameric probe based on the heptadentate gadolinium (Gd)‐AAZTA (6‐amino‐6‐methylperhydro‐1,4‐diazepinetetraacetic acid) that is able to in vivo detect PCa through the recognition of the fibrin–fibronectin (FB–FN) complex. Methods The peptide CREKA (Cys‐Arg‐Glu‐Lys‐Ala), targeting the FB–FN complex in the reactive stroma of the tumor, was synthesized by solid phase peptide synthesis (SPPS) and conjugated to the tetramer dL‐(Gd‐AAZTA) 4 . The resulting probe was characterized by 1 H relaxometry, tested in vitro on FB clots and in vivo on an orthotopic mouse model of PCa. Results CREKA‐dL‐(Gd‐AAZTA) 4 showed a remarkable relaxivity of 18.2 m M Gd - 1s−1 (0.47 T, 25°C) because of the presence of 2 water molecules (q = 2) in the inner coordination sphere of each Gd 3+ ion, whose rotational motion (τ R ) is lengthened as the result of the relatively high molecular weight. The probe displayed a detectable affinity for plasma‐derived FB clots. On intravenous injection of the probe in an orthotopic mouse model of PCa, a significant increase in the prostate T 1 contrast (~40%) was observed. The MRI signal appears statistically higher either with respect to the one observed for the control probes and to the one detected when CREKA‐dL‐(Gd‐AAZTA) 4 was administered to healthy animals. Conclusions This study demonstrated the ability of the CREKA‐dL‐(Gd‐AAZTA) 4 probe to specifically localize in prostate tumor after injection. The high relaxivity of the probe allows the reduction of the injected dose to 20 µmol Gd /kg, yielding a good in vivo contrast enhancement in the region of prostate tumor.