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Effect of body temperature on the pharmacokinetics of a triarylmethyl‐type paramagnetic contrast agent used in EPR oximetry
Author(s) -
Matsumoto Kenichiro,
Hyodo Fuminori,
Mitchell James B.,
Krishna Murali C.
Publication year - 2018
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.27008
Subject(s) - electron paramagnetic resonance , pharmacokinetics , contrast (vision) , nuclear magnetic resonance , chemistry , paramagnetism , site directed spin labeling , medicine , pharmacology , computer science , physics , quantum mechanics , artificial intelligence
Purpose Pharmacokinetics of the tri[8‐carboxy‐2,2,6,6‐tetrakis(2‐hydroxymethyl)benzo[1,2‐ d :4,5‐ d ’]bis(1,3)dithio‐4‐yl]methyl radical (Oxo63) after a single bolus and/or continuous intravenous infusion was investigated in tumor‐bearing C3H mice with or without body temperature control while under anesthesia. Method The in vivo time course of Oxo63 in blood was measured using X‐band electron paramagnetic resonance spectroscopy. Distribution of Oxo63 in normal muscle and tumor tissues was obtained using a surface coil resonator and a 700‐MHz electron paramagnetic resonance spectrometer. The whole‐body distribution of Oxo63 was obtained by 300‐MHz continuous‐wave electron paramagnetic resonance imaging. The high‐resolution 300‐MHz time‐domain electron paramagnetic resonance imaging was also carried out to probe the distribution of Oxo63. Results Urination of mice was retarded at low body temperature, causing the concentration of Oxo63 in blood to attain high levels. However, the concentration of Oxo63 in tumor tissue was lower with no control of body temperature than active body temperature control. The nonsystemized blood flow in the tumor tissues may pool Oxo63 at lower body temperature. Conclusions Pharmacokinetics of the contrast agent were found to be significantly affected by body temperature of the experimental animal, and can influence the probe distribution and the image patterns. Magn Reson Med 79:1212–1218, 2018. © Published 2017. This article is a U.S. Government work and is in the public domain in the USA