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Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2‐ 13 C]dihydroxyacetone
Author(s) -
MarcoRius Irene,
von Morze Cornelius,
Sriram Renuka,
Cao Peng,
Chang GeneYuan,
Milshteyn Eugene,
Bok Robert A.,
Ohliger Michael A.,
Pearce David,
Kurhanewicz John,
Larson Peder E.Z.,
Vigneron Daniel B.,
Merritt Matthew
Publication year - 2017
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.26525
Subject(s) - dihydroxyacetone , dihydroxyacetone phosphate , fructose , glycerol , bolus (digestion) , chemistry , medicine , carbohydrate metabolism , endocrinology , glycolysis , in vivo , metabolism , biochemistry , biology , phosphate , microbiology and biotechnology
Purpose To investigate acute changes in glucose metabolism in liver and kidneys in vivo after a bolus injection of either fructose or glucose, using hyperpolarized [2‐ 13 C]dihydroxyacetone. Methods Spatially registered, dynamic, multislice MR spectroscopy was acquired for the metabolic products of [2‐ 13 C]dihydroxyacetone in liver and kidneys. Metabolism was probed in 13 fasted rats at three time points: 0, 70, and 140 min. At 60 min, rats were injected intravenously with fructose (n = 5) or glucose (n = 4) at 0.8 g/kg to initiate acute response. Controls (n = 4) did not receive a carbohydrate challenge. Results Ten minutes after fructose infusion, levels of [2‐ 13 C]phosphoenolpyruvate and [2‐ 13 C]glycerol‐3‐phosphate halved in liver: 51% ( P = 0.0010) and 47% ( P = 0.0001) of baseline, respectively. Seventy minutes later, levels returned to baseline. The glucose challenge did not alter the signals significantly, nor did repeated administration of the dihydroxyacetone imaging bolus. In kidneys, no statistically significant changes were detected after sugar infusion other than a 20% increase of the glycerol‐3‐phosphate signal between 10 and 80 min after fructose injection ( P = 0.0028). Conclusion Hyperpolarized [2‐ 13 C]dihydroxyacetone detects a real‐time, transient metabolic response of the liver to an acute fructose challenge. Observed effects possibly include ATP depletion and changes in the unlabeled pool sizes of glycolytic intermediates. Magn Reson Med 77:65–73, 2017. © 2016 International Society for Magnetic Resonance in Medicine