Premium
Diabetes induced renal urea transport alterations assessed with 3D hyperpolarized 13 C, 15 N‐Urea
Author(s) -
Bertelsen Lotte B.,
Nielsen Per M.,
Qi Haiyun,
Nørlinger Thomas S.,
Zhang Xiaolu,
StødkildeJørgensen Hans,
Laustsen Christoffer
Publication year - 2017
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.26256
Subject(s) - urea , chemistry , renal function , diabetes mellitus , medicine , endocrinology , kidney , creatinine , nuclear magnetic resonance , biochemistry , physics
Purpose In the current study, we investigated hyperpolarized urea as a possible imaging biomarker of the renal function by means of the intrarenal osmolality gradient. Methods Hyperpolarized three‐dimensional balanced steady state 13 C MRI experiments alongside kidney function parameters and quantitative polymerase chain reaction measurements was performed on two groups of rats, a streptozotocin type 1 diabetic group and a healthy control group. Results A significant decline in intrarenal steepness of the urea gradient was found after 4 weeks of untreated insulinopenic diabetes in agreement with an increased urea transport transcription. Conclusion MRI and hyperpolarized [ 13 C, 15 N]urea can monitor the changes in the corticomedullary urea concentration gradients in diabetic and healthy control rats. Magn Reson Med 77:1650–1655, 2017. © 2016 International Society for Magnetic Resonance in Medicine