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A multisample dissolution dynamic nuclear polarization system for serial injections in small animals
Author(s) -
Krajewski Marcin,
Wespi Patrick,
Busch Julia,
Wissmann Lukas,
Kwiatkowski Grzegorz,
Steinhauser Jonas,
Batel Michael,
Ernst Matthias,
Kozerke Sebastian
Publication year - 2017
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.26147
Subject(s) - dissolution , cryostat , reproducibility , polarization (electrochemistry) , in vivo , nuclear magnetic resonance , liquid helium , materials science , chemistry , analytical chemistry (journal) , helium , chromatography , superconductivity , physics , microbiology and biotechnology , organic chemistry , quantum mechanics , biology
Purpose Several in vivo applications of dissolution dynamic nuclear polarization (DNP) require rapid successive injections of hyperpolarized substrates. Here we present the design and performance of a custom‐built multisample dissolution DNP setup for small animal research. Methods The DNP setup consists of a commercial wide‐bore magnet charged to 3.35 T, a cryostat, a 94‐GHz microwave source, and a custom‐built skeleton that accommodates four identical sample sticks. Each sample stick features a dissolver locked into the skeleton port and a lifter, which permits moving the sample cup out of the liquid helium bath for dissolution. Results The dissolution of the first sample was triggered after 2 hours of polarization buildup during single‐shot operation of the cryostat. Thereafter, a time window of 75–90 min was available to dissolve the remaining three polarized samples. The average liquid state polarization over all four sticks was measured as 18.7% ± 2.3% for [1‐13C] pyruvate 30 s after dissolution. In vivo applicability of the setup using serial injections of [1‐13C] pyruvate to study cardiac metabolism in rats revealed good reproducibility. Conclusion The proposed four‐sample DNP insert provides reproducible liquid state polarization of [1‐13C] pyruvate and allows for rapid repeat injections in small animals. Magn Reson Med 77:904–910, 2017. © 2016 International Society for Magnetic Resonance in Medicine