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Long‐term distribution of biodegradable microparticles in rat muscle quantified noninvasively by MRI
Author(s) -
Tcheudji Jacques Kameni,
Cannet Catherine,
Gérard Christelle,
Curdy Catherine,
Beckmann Nicolau
Publication year - 2016
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.25779
Subject(s) - microparticle , in vivo , biomedical engineering , plga , diffusion mri , magnetic resonance imaging , microsphere , histology , edema , chemistry , medicine , pathology , radiology , in vitro , surgery , biology , biochemistry , microbiology and biotechnology , chemical engineering , astrobiology , engineering
Purpose To demonstrate the feasibility of MRI to monitor longitudinally the fate of PLGA microparticles in muscle tissue after intramuscular injection in rats using standard equipment. Methods MRI was performed at different time points and until day 28 after intramuscular administration of microparticles. Image segmentation was used to quantify the MRI signals. Histology was performed at selected time points to validate the in vivo observations. The SOM230‐long acting release formulation was used as test compound. Results Microparticles were detected in vivo until 28 days following their administration. Imaging and histology data indicated that the MRI signals followed three phases: in an early phase (≤ 48 h after injection), vehicle, edema and hydration of microparticles contributed to the signals. In the second (days 3–17) and third phases (day 17 onward), microparticle hydration was the main contributor. SOM230 in blood displayed peaks at days 2 and 17. Conclusion MRI was suitable to follow longitudinally the presence of PLGA microparticles in the rat muscle without labeling them. This is advantageous, because labeling could potentially alter the properties and pharmacokinetics of the microparticles. Data were consistent with an initial compound release followed by diffusion and microparticle erosion as main mechanisms of SOM230 release. Magn Reson Med 75:1736–1742, 2016. © 2015 Wiley Periodicals, Inc.