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Tumor‐specific expression and detection of a CEST reporter gene
Author(s) -
Minn Il,
BarShir Am,
Yarlagadda Keerthi,
Bulte Jeff W. M.,
Fisher Paul B.,
Wang Hao,
Gilad Assaf A.,
Pomper Martin G.
Publication year - 2015
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.25748
Subject(s) - in vivo , in vitro , microbiology and biotechnology , glioma , reporter gene , gene , cell culture , gene expression , genetic enhancement , biology , cancer research , chemistry , biochemistry , genetics
Purpose To develop an imaging tool that enables the detection of malignant tissue with enhanced specificity using the exquisite spatial resolution of MRI. Methods Two mammalian gene expression vectors were created for the expression of the lysine‐rich protein (LRP) under the control of the cytomegalovirus (CMV) promoter and the progression elevated gene‐3 promoter (PEG‐3 promoter) for constitutive and tumor‐specific expression of LRP, respectively. Using those vectors, stable cell lines of rat 9L glioma, 9L CMV‐LRP and 9L PEG‐LRP , were established and tested for CEST contrast in vitro and in vivo . Results 9L PEG‐LRP cells showed increased CEST contrast compared with 9L cells in vitro . Both 9L CMV‐LRP and 9L PEG‐LRP cells were capable of generating tumors in the brains of mice, with a similar growth rate to tumors derived from wild‐type 9L cells. An increase in CEST contrast was clearly visible in tumors derived from both 9L CMV‐LRP and 9L PEG‐LRP cells at 3.4 ppm. Conclusion The PEG‐3 promoter:LRP system can be used as a cancer‐specific, molecular‐genetic imaging reporter system in vivo . Because of the ubiquity of MR imaging in clinical practice, sensors of this class can be used to translate molecular‐genetic imaging rapidly. Magn Reson Med 74:544–549, 2015. © 2015 Wiley Periodicals, Inc.