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Utilizing magnetization transfer imaging to investigate tissue remodeling in a murine model of autosomal dominant polycystic kidney disease
Author(s) -
Kline Timothy L.,
Irazabal Maria V.,
Ebrahimi Behzad,
Hopp Katharina,
Udoji Kelly N.,
Warner Joshua D.,
Korfiatis Panagiotis,
Mishra Prasanna K.,
Macura Slobodan I.,
Venkatesh Sudhakar K.,
Lerman Lilach O.,
Harris Peter C.,
Torres Vicente E.,
King Bernard F.,
Erickson Bradley J.
Publication year - 2016
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/mrm.25701
Subject(s) - magnetization transfer , magnetic resonance imaging , autosomal dominant polycystic kidney disease , medicine , kidney , pathology , polycystic kidney disease , kidney disease , nuclear medicine , radiology , disease
Purpose Noninvasive imaging techniques that quantify renal tissue composition are needed to more accurately ascertain prognosis and monitor disease progression in polycystic kidney disease (PKD). Given the success of magnetization transfer (MT) imaging to characterize various tissue remodeling pathologies, it was tested on a murine model of autosomal dominant PKD. Methods C57Bl/6 Pkd1 R3277C mice at 9, 12, and 15 months were imaged with a 16.4T MR imaging system. Images were acquired without and with RF saturation in order to calculate MT ratio (MTR) maps. Following imaging, the mice were euthanized and kidney sections were analyzed for cystic and fibrotic indices, which were compared with statistical parameters of the MTR maps. Results The MTR‐derived mean, median, 25th percentile, skewness, and kurtosis were all closely related to indices of renal pathology, including kidney weight/body weight, cystic index, and percent of remaining parenchyma. The correlation between MTR and histology‐derived cystic and fibrotic changes was R 2 = 0.84 and R 2 = 0.70, respectively. Conclusion MT imaging provides a new, noninvasive means of measuring tissue remodeling PKD changes and may be better suited for characterizing renal impairment compared with conventional MR techniques. Magn Reson Med 75:1466–1473, 2016. © 2015 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance.